16S rDNA Sequencing
16S rDNA Sequencing
The V3-V4 region is highly variable and effective in revealing diverse microbial populations, making it ideal for microbial identification and classification. With PE300 read length and high-speed capabilities, DNBSEQ-G99 is a perfect sequencer for this application.
Workflow
Sample Prep
~2 hrs
Library Prep
~6 hrs
Sequencing
~12-30 hrs
Analysis
~0.5 hr
Analysis
. MetaSIS Software
. MegaBOLT Workstation
~0.5 hr
Ordering Information
| Workflow | Type | Product name | Catalog No. |
|---|---|---|---|
| Sample Preparation | Instrument | SP-NE32 Automated Nucleic Acid Extractor | 950-000020-00 |
| Reagents | MGIEasy Stool Microbiome DNA Extraction Kit II | 940-001247-00 (96RXN) | |
| Library Preparation | Instrument | SP-960 High-throughput Automated Sample Preparation System (Config. 9) | 900-000154-00 |
| Reagents | ATOPlex 16S V3V4 rDNA Library Preparation Set | 940-001261-00 (96RXN) 940-000725-00 (576RXN) |
|
| ATOPlex E450 Dual Barcode Balanced Library Reagent | 940-000637-00 | ||
| DNBSEQ Dual Barcode Circularization Kit | 940-001310-00 (16RXN) 940-001309-00 (96RXN) |
||
| Sequencing | Instrument | DNBSEQ-G99 Genetic Sequencer | 900-000713-00 |
| Reagents | App-D FCL PE300* High-throughput Sequencing Set | 940-001717-00 | |
| Analysis | Software | MetaSIS Software (Metabarcoding Species Identification) | 970-000417-00 (unlimited) 970-000456-00 (96Report) |
| Hardware | MegaBOLT Bioinformatics Analysis Accelerator (Workstation Server) | 900-000677-00 |