Go Optical

High-Performance Imaging for Stereo-seq Workflows

Go Optical

High-Performance Imaging for Stereo-seq Workflows

High Resolution

12-megapixel imaging enables accurate segmentation at tissue and single-cell level.

Large Travel Range

Motorized XY travel range of 100 mm x 70 mm, ensuring flexibility for diverse tissue types.

Fast Imaging

Scan a 10 mm x 10 mm chip in under 70 seconds using auto-focus and a 10x objective lens.  

Go Optical is a STOmics microscope specifically designed to meet the imaging needs of Stereo-seq workflows. It employs multiple illumination techniques, including epifluorescence and transmitted bright-field microscopy, with DAPI, FITC, TRITC, and CY5 fluorescence channels. This advanced system enables efficient whole-tissue scanning and imaging, ensuring high-quality data acquisition for spatial biology applications.

Go Optical captures high-resolution images with minimal stitching errors, making it ideal for tissue segmentation in Stereo-seq applications. With a large scanning range, rapid imaging speed, clear image quality, and minimal stitching errors, it is the ideal choice for all Stereo-seq imaging applications. Equipped with an auto-focus mode, Go Optical can scan 10 mm x 10 mm chip within 70 seconds. By combining high-resolution imaging with advanced algorithms in the Stereo-seq analysis pipeline, it enables precise localization of gene expression on tissue sections.

High Resolution

 12-megapixel super-resolution imaging accurately captures track lines and tissue details, enabling precise segmentation at both the tissue and single-cell levels. 

Fast Imaging

With an unmatched imaging speed, the system scans a 10 mm × 10 mm chip in under 70 seconds using auto-focus mode and a 10X lens.

Large Travel Range 

A motorized XY travel range of 100 mm × 70 mm accommodates the scanning needs of most STOmics Stereo-seq chips, ensuring flexibility for various sample sizes. 

Applications

Go Optical imaging has various applications, including optimizing permeabilization conditions, mapping cell nuclei, and analyzing protein and tissue morphology through fluorescence photography for subsequent bioinformatics analysis. 

Permeabilization Optimalization

Imaging can help determine the optimal permeabilization time by assessing the strength of tissue fluorescence signals across different chips processed at varying time points.  

Spatial Mapping of Cell Nuclei

Fluorescence photography enables the precise recording of
cell nuclei positions and tissue morphology for downstream
bioinformatics analysis. This allows for high-resolution
visualization of cellular and tissue structures, facilitating
advanced techniques such as spatial mapping and
transcriptomic profiling.

Technical Specifications

Go Optical
Optical SystemPositive scanning microscope
Objective Lens4X, 10X, 20X, 40X (optional) universal motorized objective lens converter, support expansion
Illumination MethodsEpi-fluorescence, Epi-bright field, Transmitted bright field
Fluorescent ChannelStandard configuration includes DAPI, FITC, TRITC, CY5
Optional configuration can support other dye filter blocks
Motorized Travel Range XY100 mm x 70 mm
Camera resolutionHigh image resolution: 3008 pixels (height) and 4112 pixels (width)
Auto-focus modesAuto-focus mode and map navigation mode
Imaging stitching accuracy≤5 μm (10X)
Pixel size3.45 μm
Scanning Speed≤ 70 seconds for a 10 mm × 10 mm chip (10X)
Operating modeManual operation for human eye observation
Fully automatic scanning and imaging

Ordering Information

CategoryProductCat. No.
InstrumentSTOmics Microscope- Go Optical900-001019-00
AccessoriesObjective Lens (40X)060-000217-00