DNBSEQ Fast FS Library Prep Set V2.0

The DNBSEQ Fast FS Library Kit V2.0 is designed for preparing DNA libraries compatible with Complete Genomics DNBSEQ sequencers. This automation-friendly kit streamlines the library preparation process by combining fragmentation, end-repair, A-tailing and cleanup into a single step, significantly reducing overall time (~2 hrs) while ensuring high-quality results. Compatible with a wide DNA input range (1 – 1,000 ng) and multiple sample types for ultimate flexibility.

This kit provides increased compatibility across a wide range of applications: WGS (human/animal/plant (30x), microbial), WES, amplicon sequencing, and metagenomic sequencing.

This kit requires purchase of either the Dual Barcode Circularization Kit or the DNBSEQ OneStep Make DNB Reagent Kit V4.0.

Specifications
Product Name DNBSEQ Fast FS Library Prep Set V2.0
RXNs 16 RXN, 96 RXN, 192 RXN
Sample Type gDNA from blood, saliva, oral swabs, tissue, microbial gDNA, metagenomic DNA, plants
Input Quantity 1 ~ 1,000 ng
Insert Size Single-sided bead purification: 300 – 2,000 bp
Dual-sided bead purification: 450 – 550 bp
Barcode 192 Unique Dual Barcodes
Assay Time ~2.0 hours
Hands-On Time ~20 minutes
Automated Library Prep SP-100 (16 RXN)
Smart 8 (48 RXN)
SP-960 (96 RXN)
Read Length SE100
PE100
PE150
Sequencing Platforms DNBSEQ-E25, DNBSEQ-G99, DNBSEQ-G400, DNBSEQ-T7, DNBSEQ-T20x2
Species Category Human, animal, plant, microbe
Applications Human WGS, plant & animal WGS, microbial WGS, WES, metagenomic sequencing, amplicon sequencing

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