Single-cell Spatial RNA Sequencing to Understand Herpesviruses in Alzheimer’s Disease Using Cerebral Organoids
We have been studying the transcriptomic effects of herpesviruses such as herpes simplex virus 1 (HSV-1) in contributing to Alzheimer’s disease using human cerebral organoids. We compared our findings from single-cell spatial RNA sequencing using Stereo-seq performed on infected versus uninfected 3D cerebral organoids, as well as single-cell non-spatial RNA sequencing performed on dissociated cells from infected and uninfected cerebral organoids (2D organoids). Despite the lower infection rates in 3D organoids versus 2D organoids, we observed interesting cell type tropism in the 3D infected organoids versus 2D infected organoids. Our results illustrate some advantages of using a sequencing-based spatial transcriptomics technology for novel discoveries and data integration of multi-transcriptomics datasets.
