Complete NGS Solutions for Microbiology
Fast, Cost-Effective, Comprehensive
Complete Genomics offers a range of end-to-end NGS solutions for microbiology applications in clinical research, veterinary science, food safety, and environmental testing.
16S rDNA Sequencing
The V3-V4 region is highly variable and effective in revealing diverse microbial populations, making it ideal for microbial identification and classification. With PE300 read length and high-speed capabilities, DNBSEQ-G99 is a perfect sequencer for this application.
Workflow
Sample Prep
~2 hrs
Library Prep
~6 hrs
Sequencing
~12-30 hrs
Analysis
~0.5 hr
Sequencing
. DNBSEQ-G99 Sequencer
. App-D FCL PE300 Sequencing Set*
~12-30 hrs
Analysis
. MetaSIS Software
. MegaBOLT Workstation
~0.5 hr
*Available Q3 2024
Ordering Information
| Workflow | Type | Product name | Catalog No. |
|---|---|---|---|
| Sample Preparation | Instrument | SP-NE32 Automated Nucleic Acid Extractor | 950-000020-00 |
| Reagents | MGIEasy Stool Microbiome DNA Extraction Kit II | 940-001247-00 (96RXN) | |
| Library Preparation | Instrument | SP-960 High-throughput Automated Sample Preparation System (Config. 9) | 900-000154-00 |
| Reagents | ATOPlex 16S V3V4 rDNA Library Preparation Set | 940-001261-00 (96RXN) 940-000725-00 (576RXN) |
|
| ATOPlex E450 Dual Barcode Balanced Library Reagent | 940-000637-00 | ||
| DNBSEQ Dual Barcode Circularization Kit | 940-001310-00 (16RXN) 940-001309-00 (96RXN) |
||
| Sequencing | Instrument | DNBSEQ-G99 Genetic Sequencer | 900-000713-00 |
| Reagents | App-D FCL PE300* High-throughput Sequencing Set | 940-001717-00 | |
| Analysis | Software | MetaSIS Software (Metabarcoding Species Identification) | 970-000417-00 (unlimited) 970-000456-00 (96Report) |
| Hardware | MegaBOLT Bioinformatics Analysis Accelerator (Workstation Server) | 900-000677-00 |
Pathogen Targeted Sequencing
Pathogen Targeted Sequencing (tNGS), an amplicon-based sequencing, offers fast and cost-effective solutions for rapid screening and identification of common or known pathogens. The tNGS workflow, powered by Complete Genomics’ DNBSEQ-E25 sequencer with the AccuGen* Pathogen kits and software, enables the detection and quantification of 330 common pathogens (178 bacteria, 96 viruses, 56 fungi, and protozoans) and 150 drug-resistant genes from a variety of specimens, including blood, swab, sputum, BAL, urine, CSF, etc.
Workflow
Sample Prep
~1.5 hrs
Library Prep
~6 hrs
Sequencing
~3 hrs
Analysis
~0.5 hr
Sample Prep
~1.5 hrs
Library Prep
~6 hrs
Sequencing
~3 hrs
Analysis
~0.5 hr
*Complete Genomics is the sole distributor of AccuGen products, from Health GeneTech (HGT), in the US.
Ordering Information
| Workflow | Type | Product name | Catalog No. |
|---|---|---|---|
| Sample Preparation | Reagents | AccuGen* Pathogen DNA/RNA Extraction Kit | 961-000013-00 (50 tests) |
| Library Preparation | Reagents | AccuGen* Pathogen Multiplex-PCR Library Prep Kit | 961-000014-00 (96 tests) |
| Sequencing | Instrument | DNBSEQ-E25 Genetic Sequencer | 900-000703-00 |
| Reagents | FCL SE100 High-throughput Sequencing Set | 940-000894-00 | |
| Analysis | Software | AccuGen Rapid Identification of Pathogen Microorganisms Software | Pre-installed on DNBSEQ-E25 |
Shotgun Metagenomics Sequencing
Shotgun metagenomic sequencing (mNGS), is a powerful tool for gaining comprehensive insights into the biodiversity and functions of entire microbial communities directly from natural environments such as soil, water, or the human gut. It is espe-cially effective for studying unculturable microorganisms, which traditional methods cannot analyze. Complete Genomics offers automated end-to-end mNGS solutions with various library preparation systems and sequencers tailored to your throughput needs.
Workflow
Sample Prep
Library Prep
Sequencing
Analysis
Sample Prep
Library Prep
Sequencing
Analysis
*Available Q3 2024 **Available Q4 2024
Ordering Information
| Workflow | Type | Product name | Catalog No. |
|---|---|---|---|
| Sample Preparation | Instrument | SP-NE32 Automated Nucleic Acid Extractor | 950-000020-00 |
| Reagents | MGIEasy Nucleic Acid Extraction Kit | 1000023774 (32 RXN) | |
| MGIEasy Microbiome DNA Extraction Kit | 1000027955 (96 RXN) | ||
| MGIEasy Stool Microbiome DNA Extraction Kit II | 940-001247-00 (96 RXN) | ||
| Library Preparation | Instrument | SP-100 Automated Sample Preparation System | 900-000206-00 |
| Reagents | DNBSEQ Fast FS Library Prep Kit V2.0 | 940-001511-00 (16 RXN) 940-001509-00 (96 RXN) |
|
| DNBSEQ OneStep Make DNB Reagent Kit V4.0 | 940-001750-00 (Dual Barcode, 4RXN) | ||
| Sequencing | Instrument | DNBSEQ-G99 Genetic Sequencer | 900-000713-00 |
| Reagents | FCL SE100/PE50 High-Throughput Sequencing Set | 940-000904-00 | |
| FCL PE150 High-Throughput Sequencing Set | 940-000905-00 | ||
| Analysis | Software | PFI Pro (Platform of Microorganisms Fast Identification and Assembly Evolution) | 900-000399-00 |
| Workflow | Type | Product name | Catalog No. |
|---|---|---|---|
| Sample Preparation | Instrument | STP-7000 High-Throughput Automated Sample Transfer System | 900-000336-00 |
| SP-960 High-Throughput Automated Sample Preparation System (Config. 9) | 900-000154-00 | ||
| Reagents | MGIEasy Stool Microbiome Extraction Kit II | 940-001247-00 (96 RXN) 940-001246-00 (384 RXN) |
|
| Library Preparation | Instrument | SP-960 High-Throughput Automated Sample Preparation System (Config. 7) | 900-000152-00 |
| Reagents | DNBSEQ Fast FS Library Prep V2.0 Kit | 940-001509-00 (96RXN) | |
| QC / Normalization / Pooling | Instrument | SP-Smart 8 Automated Liquid Handling System (Config. 4) | 900-000495-00 |
| Sequencing | Instrument | DNBSEQ-G400 Genetic Sequencer | 900-000641-00 |
| Reagents | FCL PE150 High-Throughput Sequencing Set | 940-000810-00 | |
| Analysis | Hardware | ZTRON Lite Data Center | 900-000673-00 |
| Software | PFI Pro (Platform of Microorganisms Fast Identification and Assembly Evolution) | 900-000399-00 |
| Workflow | Type | Product name | Catalog No. |
|---|---|---|---|
| Sample Preparation | Instrument | STP-7000 High-Throughput Automated Sample Transfer System | 900-000336-00 |
| SP-960 High-Throughput Automated Sample Preparation System (Config. 9) | 900-000154-00 | ||
| Reagents | MGIEasy Stool Microbiome Extraction Kit II | 940-001247-00 (96 RXN) 940-001246-00 (384 RXN) |
|
| Library Preparation | Instrument | SP-960 High-Throughput Automated Sample Preparation System (Config. 7) | 900-000152-00 |
| Reagents | DNBSEQ Fast FS Library Prep V2.0 Kit | 940-001509-00 (96RXN) | |
| QC / Normalization / Pooling | Instrument | SP-Smart 8 Automated Liquid Handling System (Config. 4) | 900-000495-00 |
| Sequencing | Instrument | DNBSEQ-T7 Genetic Sequencer | 900-000698-00 |
| Reagents | FCL PE150 High-Throughput Sequencing Set | 940-000836-00 | |
| Analysis | Hardware | ZTRON Lite Data Center | 900-000673-00 |
| Software | PFI Pro (Platform of Microorganisms Fast Identification and Assembly Evolution) | 900-000399-00 |
3rd Party Kit Compatibility
DNBSEQ-E25 Sequencer
| Sequencing Reagent | Catalog No. |
|---|---|
| App-C FCL SE100 High-throughput Sequencing Set | 940-000895-00 |
| App-C FCL PE150 High-throughput Sequencing Set | 940-000901-00 |
DNBSEQ-G99 Sequencer
| Library Conversion Kits | Catalog No. |
|---|---|
| OneStep Library Conversion Kit V4.0 | 940-001648-00 |
| DNBSEQ Universal Library Conversion Kit | 940-000963-00 |
DNBSEQ-G400 Sequencer
You need to order only one of these kits.
| Library Conversion Kits | Catalog No. |
|---|---|
| OneStep Library Conversion Kit V4.0 | 940-001648-00 |
| DNBSEQ Universal Library Conversion Kit | 940-000963-00 |
You need to order both of the reagents.
| Sequencing Reagent | Catalog No. |
|---|---|
| FCL PE150 High-throughput Sequencing Set | 940-000810-00 |
| App-D PE Primer Kit | 940-000917-00 |
DNBSEQ-T7 Sequencer
You need to order only one of these kits.
| Library Conversion Kits | Catalog No. |
|---|---|
| OneStep Library Conversion Kit V4.0 | 940-001648-00 |
| DNBSEQ Universal Library Conversion Kit | 940-000963-00 |
You need to order both of the reagents.
| Sequencing Reagent | Catalog No. |
|---|---|
| FCL PE150 High-throughput Sequencing Set | 940-000836-00 |
| App-D PE Primer Kit | 940-000917-00 |