Type of Extraction

MGIEasy Total RNA Extraction Set

Extract RNA from samples using high-concentration salt ion lyse with magnetic bead capture and wash buffer to remove proteins. Suitable for total RNA extraction from samples such as cells, animal tissues (fresh or frozen), blood, and more. Compatible with SP-960 and SP-NE384 automation platforms.

P/N940-000880-00 (96 RXN), 940-000875-00 (384 RXN)
SpeciesHuman, Animal, Bacteria
Sample typeEukaryotic cells, humans or animal tissues, blood, and prokaryotic cells such as bacterial G+ and bacterial G -.
Input volumeHuman blood: 100 ~ 200 μL
Animal tissue: 1 ~ 20 mg (manual); 1 – 20 mg (SP-NE32); 5 – 30 mg (SP-NE384); 1 – 30 mg (SP-960)
Cell: 1 × 10⁵ ~ 5 × 10⁶
Bacteria :5 × 10⁷ ~ 5 × 10⁹
Yeast: 5 × 10⁷
Elution volume50 – 150 μL
Assay timeManual: 40 min
SP-NE32: 50 min
SP-NE384: 60 min
SP-960: 90 min
ApplicationRNA-Seq, RT-PCR, and more
Lab auto platformSP-NE32, SP-NE384, SP-960

DNBSEQ rRNA Depletion Kit

Efficiently deplete rRNA (including cytoplasmic 5 S rRNA, 5.8 S rRNA, 18 S rRNA, 28 S rRNA, mitochondrial ribosomal RNA 12 S rRNA, 16 S rRNA and 45 S pre-ribosomal RNA) from human, mouse and rat samples using a low input of 10 ng~1 μg total RNA. The resulting mRNA and other noncoding RNA are suitable for downstream RNA analysis applications. This kit is compatible with both intact and degraded RNA (e.g. FFPE RNA).

P/N940-001812-00 (16 RXN), 940-001811-00 (96 RXN)
SpeciesHuman, Mouse, Rat
Sample typeTotal RNA from blood fresh tissue, FFPE RNA
Input volumeTotal RNA: 10 ~ 1 μg
Elution volumeVariable depending on application
Assay time2 hrs
ApplicationWhole-Transcriptome Sequencing, Long Non-Coding RNA Sequencing
Lab auto platformSP-100, SP-960

DNBSEQ rRNA & Globin Depletion Kit

Efficiently deplete rRNA and Globin mRNA (including cytoplasmic 5 S rRNA, 5.8 SrRNA, 18 S rRNA, 28 S rRNA, mitochondrial ribosomal RNA 12 S rRNA, 16 S rRNA and 45 S preribosomal RNA) and Globin mRNA (including HBA1/2, HBB, HBD, HBG1/2, HBM, HBE1,HBQ1,HBZ) from human blood using a low input of 10 ng~1 μg total RNA. The resulting mRNA and other noncoding RNA are suitable for downstream RNA analysis applications.

P/N940-001810-00 (16 RXN), 940-001813-00 (96 RXN)
SpeciesHuman
Sample typeTotal RNA from Human blood
Input volumeTotal RNA: 10 ~ 1 μg
Elution volumeVariable depending on application
Assay time2 hrs
ApplicationWhole-Transcriptome Sequencing, Long non-coding RNA Sequencing
Lab auto platformSP-100, SP-960

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