DNBSEQ™ Fast RNA Library Prep Set
The DNBSEQ Fast RNA Library Kit is designed for preparing RNA libraries for the Complete Genomics DNBSEQ sequencers. Compatible with a wide RNA input range (10 ng – 1 ug) and multiple sample types for ultimate flexibility.
This automation-friendly kit streamlines the library preparation process by combining cDNA second strand synthesis and end-repair steps, significantly reducing overall time (~4 hrs) while ensuring high-quality results. This kit also includes additional flexibility in the second strand synthesis step. It comes with two buffer options (dNTP buffer and dUTP buffer) allowing users the option to either prepare a directional or non-directional library.
This kit requires purchase of either the Dual Barcode Circularization Kit or the DNBSEQ OneStep Make DNB Reagent Kit V4.0.
| Specifications | |
|---|---|
| Product Name | DNBSEQ Fast RNA Library Prep Set |
| RXNs | 16 RXN, 96 RXN, 192 RXN |
| Sample Type | Total (bulk) RNA from blood, saliva, fresh tissue, FFPE RNA, plant, animal, microbial, and meta |
| Input Quantity | 10 ng ~ 1 ug total RN |
| Strand Specificity | Non-stranded or stranded library prep Note: Kit comes with two buffers (dNTP buffer, dUTP buffer) to allow users to select the type of library preparation method |
| Barcode | 192 Unique Dual Barcodes |
| Assay Time | ~4.0 hours |
| Hands-On Time | ~1 hour | Automated Library Prep |
SP-100 (16 RXN) SP-Smart8 SP-960 (96 RXN, 192 RXN) |
| Read Length | SE50 PE100 PE150 |
| Sequencing Platforms | DNBSEQ-E25, DNBSEQ-G99, DNBSEQ-G400, DNBSEQ-T7, DNBSEQ-T20x2 |
| Species Category | Human, animal, plant, microbe |
| Applications | Whole-Transcriptome Sequencing, mRNA sequencing, long non-coding RNA sequencing, RNA-Seq, Meta transcriptome sequencing |
A Complete Solution for Every Step of Your Workflow
Kits & Reagents
No matter your workflow, our kits are simple to use and easily integrated. We also offer conversion kits for 3rd party library prep.
Lab Automation
With solutions from sample extraction to lab automation you can spend less time overseeing equipment and pursue more useful activities.
